2 edition of Fluorescence studies of phospholipid vesicle fusion leakage and exchange. found in the catalog.
Fluorescence studies of phospholipid vesicle fusion leakage and exchange.
Yiannakis P. Yianni
PhD thesis, Biochemistry.
of an aqueous content dye. By analyzing the diffusion proﬁle of the aqueous dye after vesicle fusion, we are able to distinguish content transfer across the tethered bilayer patch from vesicle leakage above the patch. Received for publication 29 June and in ﬁnal form 20 September *Correspondence: [email protected] Jun 15, · Mechanism of Formation of Vesicle Fused Phospholipid Monolayers on Alkanethiol Self-Assembled Monolayer Supports. Published. June 15, Author(s) John T. Woodward IV, Curtis W. Meuse. Citation. Journal of Colloid and Interface Science. Volume. Issue. 2. Cited by:
"The Role of the Distal and Proximal Protein Environments in Controlling the Ferric Spin State and in Stabilizing Thiolate Ligation in Heme Systems: Thiolate Adducts of the Myoglobin H93G Cavity Mutant", Mark P. Roach, Alycen E. Pond, Melissa R. Thomas, Steven G. Boxer, and John H. Dawson, Journal of the American Chemical Society, , (). The probe exhibited a higher fluorescence response to ·OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect ·OH lestisserandsduquebec.com by:
Fluorescence intensity in vesicle and cortex of the cell. Dear ImageJ community, I am having trouble analyzing the fluorescence intensity of a FRET biosensor in the cortex of the cell (please, see. Oct 13, · Structure and Membrane Interactions of Mutant HA Fusion Peptides. Structural studies of synthetic fusion peptides of the X HA have shown that the identity of N-terminal residues can affect the conformation of the peptide and its interaction with phospholipid bilayers (Gray et al., ).
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Agents such as antimicrobial peptides and toxins can permeabilize membrane vesicles to cause leakage of entrapped contents in either a graded or an all-or-none fashion.
Determination of which mode of leakage is induced is an important step in understanding Cited by: Monitoring of phospholipid vesicle fusion by fluorescence energy transfer between membrane-bound dye labels. In previous studies Ca2÷ was shown to promote leakage across phosphatidyl- serine membranes but the leakage was correlated with the onset of fusion .
Discussion The present studies show that the extent of mixing of membrane Cited by: Nov 01, · Video fluorescence microscopy was used to study adsorption and fusion of unilamellar phospholipid vesicles to solvent-free planar bilayer membranes. Large unilamellar vesicles ( microns diam) were loaded with mM of the membrane-impermeant fluorescent dye calcein.
In cell biology, a vesicle is a structure within or outside a cell, consisting of liquid or cytoplasm enclosed by a lipid lestisserandsduquebec.comes form naturally during the processes of secretion (), uptake (endocytosis) and transport of materials within the plasma lestisserandsduquebec.comatively, they may be prepared artificially, in which case they are called liposomes (not to be confused with lysosomes).
conflicting results with respect to whether fusion occurs (11, 20) and, if so, whether leakage of vesicle contents is concom- itant and/or sizable (13, 14, 18, 19). Since the calcein assay is particularly appropriate for the kinetic analysis of vesicle fusion and lysis, we felt it would be an informative probe for PS SUV studies.
A Fluorescence Assay to Monitor Vesicle Fusion and Lysis of its intense fluorescence. Leakage of either component of the reaction can be directly quantitated by titrating the external medium.
Request PDF | Molecular interactions of peptide with phospholipid vesicle membranes as studied by fluorescence correlation spectroscopy | Interactions of the peptides melittin and magainin with. Abstract. While the aqueous solubility for bilayer phospholipids is less than 10 −10 M—keeping lipid membranes at essentially constant mass, single chain surfactants can have a significant aqueous solubility.
Thus, in surfactant solutions, both monomer and micelles can interact with a lipid bilayer, and the mass and composition of the bilayer can be changed in lestisserandsduquebec.com by: Stress-Induced Leakage from Phospholipid Vesicles: Effect of Membrane Composition Scott D.
Shoemaker and T. Kyle Vanderlick* Department of Chemical Engineering, Princeton University, Princeton, New Jersey When subjected to large hypoosmotic gradients, lipid vesicles swell and rupture, expelling. Phospholipid vesicle fusion induced by cations and poly-(ethylene glycol) Poste G () Studies on membrane fusion.
Induction of fusion in pure phospholipid membranes by calcium and other divalent cations. Monitoring of phospholipid vesicle fusion by fluorescence energy transfer between membrane-bound dye labels.
Biochim Biophys Cited by: 4. Jul 01, · In this report, the mechanisms and kinetics of saposin-induced phospholipid vesicle fusion have been determined by using a particle size analyzer and fluorescence spectroscopy. Saposin C has potent fusogenic activity at nanomolar levels.
A model for saposin C-induced vesicle membrane fusion based on these studies is proposed. Materials and methodsCited by: Bio Test 1. STUDY. PLAY.
amphiphilic. Substances that have a hydrophilic and a hydrophobic end. artificial phospholipid bilayer vesicle formed from an aqueous suspension of phospholipid molecules.
fluorescence recovery after photobleaching; technique for monitoring the kinetic parameters of a protein by analyzing how fluorescent. bios chapter 5 study guide by Elizabeth_Ruzek includes 65 questions covering vocabulary, terms and more.
Quizlet flashcards, activities and games help you improve your grades. These results show that physiological levels of diacylglycerols can substantially increase the susceptibility of phospholipid membranes to fusion.
This suggests that, in addition to their role in protein kinase C activation, diacylglycerols could play a more direct role in the.
DRUG PERMEABILITY ACROSS A PHOSPHOLIPID VESICLE BASED BARRIER 2. CHARACTERIZATION OF BARRIER STRUCTURE, The permeation studies performed using the phospholipid vesicle based barrier have been conducted at pH in both the donor compartment, the liposome-barrier and the acceptor liposomes fusion to produce a tight barrier.
The cell membrane consists of three classes of amphipathic lipids: phospholipids, glycolipids, and lestisserandsduquebec.com amount of each depends upon the type of cell, but in the majority of cases phospholipids are the most abundant, often contributing for over 50% of all lipids in plasma membranes.
Use of resonance energy transfer to monitor membrane fusion. ADVERTISEMENT. Log In Register. Cart Use of resonance energy transfer to monitor membrane fusion.
Douglas K. Struck, Dick Hoekstra, and Richard E. Pagano. Lipid Exchange, and Effect of Vesicle Size in. We report a new system of laterally mobile, arrayed vesicles that are encoded with DNA to control tethering to fluid-supported phospholipid bilayers.
The motion of individual fluorescently labeled vesicles, specifically bound, are easily visualized by fluorescence video microscopy and observed to collide reversibly on the surface.
This system is an ideal model for studying interactions Cited by: the phospholipid vesicle-based permeation assay (PVPA) model was conﬁrmed by a set of differ- numerous studies have explored this effect and the resul-tant alterations in structure (Schubert et al., ; Schubert and in fusion of the liposomes so that tight barriers were Nov 14, · Based on these studies we propose a new approach to measure electrostatic potential at the surface layer of phospholipid membranes, which is based on potential‐dependent changes of bilayer hydration and involves very sensitive and convenient ratiometric measurements in fluorescence lestisserandsduquebec.com by:.
Vesicle Adsorption and Lipid Bilayer Formation on Glass of McConnell and co-workers,17,18 vesicle fusion has been * Corresponding author. E-mail: [email protected] used to form supported bilayers from various lipids on based on two color fluorescence microscopy.1 Fusion was observed by including a self-quenching concentration of.Steady-state fluorescence oxygen quenching experiments were performed on phospholipid vesicles where 2-dimethylaminolauroylnaphthalene (Laurdan) was inserted.
The quenching efficiency was found to be much higher in vesicles in the liquid-crystalline phase with respect to the gel phase, by a factor of about Cited by: Feb 22, · AbstractA full membrane fusion model which attains both complete lipid mixing and content mixing liposomal membranes mediated by coiled-coil forming lipopeptides LPK [L-PEG(KIAALKE)3] and LPE [L-PEG(EIAALEK)3] is presented.
The electrostatic effects of lipid anchored peptides on fusion efficiency was investigated. For this, the original amino acid sequence of the Author: Tingting Zheng, Yun Chen, Yu Shi, Huanhuan Feng.